Journal: Journal of cellular physiology

Article Title: Potential role of follicle-stimulating hormone (FSH) and transforming growth factor (TGFβ1) in the regulation of ovarian angiogenesis.

doi: 10.1002/jcp.22491

Figure Lengend Snippet: Fig. 7. Long-term treatment effect of VEGFR2 and PDGFR inhibitors on secreted gelatinase activity in rat aortic ring culture. Aortic ring cultures were treated as described in Figure 3, and conditioned media of aortic ring cultures were collected and analyzed by gelatin zymography. A: Characterization of gelatinases with aliquots of sample incubated without or with a metalloproteinase inhibitor (1,10-phenanthroline, 5 mM) during substrate reaction step. B and C: Representative zymograms of aortic ring cultures treated with Ki8751 and AG1296, respectively. D: Quantitative analysis of gelatinase activity by densitometry. Each bar represents the mean (WSEM) relative density (n U 4). Different lowercase letters indicate significant differences among treatment groups in the absence of inhibitor (P < 0.05). M, indicates a significant difference compared with the respective control without inhibitors (P < 0.05). D, day; IM, incubation medium control; C, control-GCCM; F, FSH-GCCM; T, TGFb1-GCCM; F R T, FSH R TGFb1-GCCM; M, protein markers.

Article Snippet: Quantification of gelatinases was achieved by computerized image analysis using two-dimensional laser scanning densitometry (Molecular Dynamics, Sunnyvale, CA).

Techniques: Activity Assay, Zymography, Incubation, Control